4ZTP
Fab structure of rabbit monoclonal antibody R53 targeting an epitope in HIV-1 gp120 C4 region
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-10 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.03330 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 105.433, 78.553, 68.480 |
| Unit cell angles | 90.00, 92.25, 90.00 |
Refinement procedure
| Resolution | 31.487 - 1.630 |
| R-factor | 0.1963 |
| Rwork | 0.195 |
| R-free | 0.22770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4jo1 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.096 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Number of reflections | 66122 | |
| <I/σ(I)> | 21.5 | 2.35 |
| Completeness [%] | 95.4 | 79 |
| Redundancy | 3.9 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 296 | 14% polyethylene glycol 8000, 0.1 M HEPES pH 7.5, and 8% ethylene glycol |
