4ZTI

Ebola virus nucleoprotein bound to VP35 chaperoning peptide P212121

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-05
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.03318
Spacegroup name	P 21 21 21
Unit cell lengths	94.881, 94.951, 112.106
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.270 - 2.400
R-factor	0.1946
Rwork	0.192
R-free	0.24700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zta
RMSD bond length	0.014
RMSD bond angle	1.482
Data reduction software	MOSFLM
Data scaling software	Aimless (0.1.27)
Phasing software	PHASER (2.5.7)
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.270	48.270	2.490
High resolution limit [Å]	2.400	8.980	2.400
Rmerge	0.151	0.091	1.556
Rpim	0.060	0.039	0.607
Total number of observations	298839	5566	31129
Number of reflections	40325
<I/σ(I)>	8.2	18.6	1.5
Completeness [%]	100.0	99.3	99.8
Redundancy	7.4	6.3	7.5
CC(1/2)	0.993	0.989	0.575

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.4	277	2.0 M sodium formate, 100 mM sodium acetate pH 4.4