4ZTG
Ebola virus nucleoprotein bound to VP35 chaperoning peptide P22121
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-03-26 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.0332 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 33.349, 92.398, 133.626 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.000 - 2.800 |
R-factor | 0.2625 |
Rwork | 0.259 |
R-free | 0.29330 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.593 |
Data reduction software | MOSFLM |
Data scaling software | XDS |
Phasing software | PHASER (2.5.7) |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.200 | 46.200 | 2.950 |
High resolution limit [Å] | 2.800 | 8.840 | 2.800 |
Rmerge | 0.084 | 0.033 | 1.042 |
Rpim | 0.036 | 0.015 | 0.449 |
Total number of observations | 68091 | 2132 | 9268 |
Number of reflections | 10825 | ||
<I/σ(I)> | 12.9 | 39.6 | 1.6 |
Completeness [%] | 99.7 | 99 | 98.4 |
Redundancy | 6.3 | 5.2 | 6.2 |
CC(1/2) | 0.999 | 0.999 | 0.811 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 277 | 12% PEG 6000, 100 mM MES pH 5.0 |