4ZTF

Crystal Structure of the Prototype Foamy Virus Intasome with a 2-Pyridinone Aminal Inhibitor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-01-09
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97949
Spacegroup name	P 41 21 2
Unit cell lengths	159.450, 159.450, 124.090
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.858 - 2.700
R-factor	0.1922
Rwork	0.191
R-free	0.21460
RMSD bond length	0.010
RMSD bond angle	1.100
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER-TNT (2.11.5)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.858	48.858	2.709
High resolution limit [Å]	2.700	12.463	2.700
Rmerge	0.107	0.062	0.977
Total number of observations	489046	4997	4686
Number of reflections	44469
<I/σ(I)>	14.5
Completeness [%]	100.0	100	100
Redundancy	11	9.5	11.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	1.25 M AMMONIUM SULFATE, 25% (V/V) GLYCEROL, 4.8% (V/V) 1,6-HEXANEDIOL, 50 MM MES-NAOH, 1MM EDTA, PH 6.5