4ZTA
Ebola virus nucleoprotein bound to VP35 chaperoning peptide I212121
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.70004 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 91.210, 93.140, 114.940 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 72.360 - 2.400 |
| R-factor | 0.205 |
| Rwork | 0.203 |
| R-free | 0.23340 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.566 |
| Data reduction software | XDS (November 3, 2014) |
| Data scaling software | XSCALE (November 3, 2014) |
| Phasing software | PHENIX (dev-1839) |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.260 | |
| High resolution limit [Å] | 2.200 | 9.840 | 2.200 |
| Rmerge | 0.144 | 0.055 | 4.966 |
| Rmeas | 0.145 | 0.055 | 5.013 |
| Total number of observations | 4175583 | ||
| Number of reflections | 19509 | 533 | 3595 |
| <I/σ(I)> | 27.52 | 111.4 | 1 |
| Completeness [%] | 100.0 | 99.3 | 100 |
| Redundancy | 172.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.7 | 277 | 2.2M Sodium formate, 100mM NaOAc pH 4.7 |
