4ZT8
Structure of the complex of type 1 ribosome inactivating protein from Momordica balsamina with a pyrimidine base, cytosine at 1.98 A resolution
Replaces: 4KJHExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2013-05-01 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97 |
Spacegroup name | H 3 |
Unit cell lengths | 130.258, 130.258, 39.939 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.630 - 1.980 |
R-factor | 0.17959 |
Rwork | 0.178 |
R-free | 0.20423 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3s9q |
RMSD bond length | 0.005 |
RMSD bond angle | 1.089 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.630 | 2.010 |
High resolution limit [Å] | 1.980 | 1.980 |
Rmerge | 0.283 | |
Number of reflections | 16671 | |
<I/σ(I)> | 32.3 | 4.2 |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 298 | 14% PEG 6000, 0.1M Sodium Phosphate |