4ZSX

Structure of a fusion protein with a helix linker, 2ARH-3-3KAW-2.0

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-03-01
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	H 3
Unit cell lengths	121.430, 121.430, 207.820
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	93.832 - 2.190
R-factor	0.2294
Rwork	0.228
R-free	0.24920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2arh 3kaw
RMSD bond length	0.013
RMSD bond angle	1.443
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	93.832		2.260
High resolution limit [Å]	2.190	9.840	2.190
Rmerge	0.074	0.065	0.651
Rmeas	0.081	0.071	0.718
Total number of observations	338449
Number of reflections	57982	649	4237
<I/σ(I)>	13.81	28.43	3
Completeness [%]	99.9	99.8	98.7
Redundancy	5.83

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	1.0M ammonium phosphate monobasic