4ZSV

Structure of a fusion protein with a helix linker, 2ARH-3-3KAW-1.0

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-04-23
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 63 2 2
Unit cell lengths	191.610, 191.610, 114.690
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	95.810 - 4.200
R-factor	0.3374
Rwork	0.333
R-free	0.37620
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.033
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	95.810		4.310
High resolution limit [Å]	4.200	18.790	4.200
Rmerge	0.063	0.017	0.510
Rmeas	0.065	0.018	0.523
Total number of observations	183546
Number of reflections	9491	141	669
<I/σ(I)>	35.75	127.49	7.55
Completeness [%]	99.9	99.3	99.1
Redundancy	19.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	0.9M ammonium tartrate dibasic