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4ZSN

70S-wild-type HigB toxin complex bound to a AAA lysine codon

This is a non-PDB format compatible entry.
Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 24-ID-C
Synchrotron siteAPS
Beamline24-ID-C
Temperature [K]100
Detector technologyPIXEL
Collection date2014-08-11
DetectorPSI PILATUS 6M
Wavelength(s)0.97920
Spacegroup nameP 21 21 21
Unit cell lengths213.446, 452.916, 608.938
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution49.920 - 3.600
R-factor0.1998
Rwork0.198
R-free0.23400
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.004
RMSD bond angle0.869
Data reduction softwareHKL-2000
Data scaling softwareXSCALE
Phasing softwarePHENIX
Refinement softwarePHENIX
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]49.9203.730
High resolution limit [Å]3.6003.600
Rmerge0.2160.828
Number of reflections666396
<I/σ(I)>6.61.8
Completeness [%]98.799.2
Redundancy5.184.22
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP72934% PEG 20k, 4% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN

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