4ZSH

RXR LBD in complex with 9-cis-13,14-dihydroretinoic acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-02-27
Detector	DECTRIS PILATUS 200K
Wavelength(s)	0.97625
Spacegroup name	P 43 21 2
Unit cell lengths	64.010, 64.010, 112.070
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 1.800
Rwork	0.209
R-free	0.21800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zxz
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.860
High resolution limit [Å]	1.800	1.800
Number of reflections	22407
<I/σ(I)>	31.2
Completeness [%]	99.2	97.2
Redundancy	7.2	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		290	PEG3350 18%, calcium acetate 50 mM