4ZSC
Human Cyclophilin D Complexed with an Inhibitor at room temperature
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2015-05-07 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9797 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 57.866, 57.866, 88.463 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.430 - 1.500 |
R-factor | 0.135 |
Rwork | 0.134 |
R-free | 0.16280 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 48.400 |
High resolution limit [Å] | 1.500 |
Number of reflections | 16982 |
<I/σ(I)> | 12.1 |
Completeness [%] | 73.1 |
Redundancy | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 291 | 30% PEG4000 |