4ZSB
Crystal structure of the ligand-free effector-binding domain of DasR (DasR-EBD)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-02 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 56.634, 56.634, 108.995 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.727 - 2.004 |
| R-factor | 0.2145 |
| Rwork | 0.212 |
| R-free | 0.25620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Effector-binding domain of entry 2wv0 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.101 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.080 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.054 | 0.806 |
| Number of reflections | 14018 | |
| <I/σ(I)> | 17.5 | 1.4 |
| Completeness [%] | 98.9 | 94.2 |
| Redundancy | 4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 292.15 | 0.1 M Bis-Tris propane pH 9.0, 4.0 M potassium formate, 20 % (w/v) PEG monomethyl ether 2,000 |
