4ZS6
Receptor binding domain and Fab complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-05-06 |
| Detector | RIGAKU SATURN A200 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.492, 64.461, 186.049 |
| Unit cell angles | 90.00, 100.43, 90.00 |
Refinement procedure
| Resolution | 36.953 - 3.166 |
| R-factor | 0.2055 |
| Rwork | 0.203 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4l72 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.494 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.270 |
| High resolution limit [Å] | 3.166 | 7.880 | 3.166 |
| Rmerge | 0.170 | 0.085 | 0.876 |
| Total number of observations | 156458 | ||
| Number of reflections | 31146 | ||
| <I/σ(I)> | 7.1 | ||
| Completeness [%] | 95.1 | 90.1 | 96.7 |
| Redundancy | 5 | 4.6 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.1 M MES monohydrate (pH 6.5), 12% (w/v) Polyethylene glycol 20000 |
