4ZRX
Crystal structure of a putative alpha-L-fucosidase (BACOVA_04357) from Bacteroides ovatus ATCC 8483 at 1.59 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-10 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | .979230 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.021, 71.754, 73.106 |
| Unit cell angles | 90.00, 106.98, 90.00 |
Refinement procedure
| Resolution | 28.599 - 1.590 |
| R-factor | 0.1526 |
| Rwork | 0.151 |
| R-free | 0.17520 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.648 |
| Data reduction software | XDS |
| Data scaling software | XSCALE (November 3, 2014 BUILT=20141118) |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 28.599 | 1.650 | |
| High resolution limit [Å] | 1.590 | 3.420 | 1.590 |
| Rmerge | 0.043 | 0.023 | 0.576 |
| Rmeas | 0.052 | 0.027 | 0.691 |
| Total number of observations | 514757 | ||
| Number of reflections | 81860 | 16058 | 16765 |
| <I/σ(I)> | 14.68 | 40.2 | 2 |
| Completeness [%] | 97.5 | 96.7 | 96.4 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 24.0% polyethylene glycol 3350, 0.11M potassium chloride |
