4ZRT
PTP1BC215S bound to Nephrin peptide substrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-15 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.848, 88.725, 50.043 |
| Unit cell angles | 90.00, 97.25, 90.00 |
Refinement procedure
| Resolution | 19.950 - 1.740 |
| R-factor | 0.17037 |
| Rwork | 0.168 |
| R-free | 0.20743 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.153 |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 19.950 |
| High resolution limit [Å] | 1.740 |
| Rmerge | 0.120 |
| Number of reflections | 38922 |
| <I/σ(I)> | 6.2 |
| Completeness [%] | 99.7 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 14.4% PEG 8000, 0.1 M HEPES (pH 7.5), 0.2 M MgCl2 |
