4ZRR
Crystal Structure of Monomeric Bacteriophytochrome mutant D207L Y263F at 1.5 A resolution Using a home source.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-14 |
| Detector | BRUKER SMART 6000 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 94.819, 55.046, 69.797 |
| Unit cell angles | 90.00, 91.89, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.500 |
| R-factor | 0.162 |
| Rwork | 0.160 |
| R-free | 0.19500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4o8g |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.389 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.600 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.179 | |
| Number of reflections | 54864 | |
| <I/σ(I)> | 14.69 | 4.83 |
| Completeness [%] | 96.0 | 91.2 |
| Redundancy | 2.9 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 298 | Crystals of D207L-Y263F phytochrome mutant were grown from hanging drop vapor diffusion in 20% PEG400, starting protein concentration of 20 mg/ml, and 0.1M phosphate citrate buffer at pH 4.0. |
