4ZRM

Crystal Structure of UDP-Glucose 4-Epimerase (TM0509) from Hyperthermophilic Eubacterium Thermotoga maritima

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2013-09-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 21 21 2
Unit cell lengths	82.542, 150.262, 60.606
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.972 - 1.900
R-factor	0.1584
Rwork	0.157
R-free	0.18410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2p5y
RMSD bond length	0.007
RMSD bond angle	1.149
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.930
High resolution limit [Å]	1.900	1.900
Rmerge	0.086	0.545
Number of reflections	60368
<I/σ(I)>	29.9	8.3
Completeness [%]	99.0	96.3
Redundancy	13.2	13.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	8% Tascimate pH 5.0, 20% PEG3350