4ZRL
Structure of the non canonical Poly(A) polymerase complex GLD-2 - GLD-3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-17 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97975 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.655, 89.367, 94.666 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.300 - 2.280 |
| R-factor | 0.1892 |
| Rwork | 0.187 |
| R-free | 0.23050 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.753 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELXDE |
| Refinement software | PHENIX (dev_1615) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.300 | 2.420 |
| High resolution limit [Å] | 2.280 | 2.283 |
| Rmerge | 0.731 | |
| Number of reflections | 37155 | |
| <I/σ(I)> | 13.36 | 3.03 |
| Completeness [%] | 98.4 | 90.6 |
| Redundancy | 7.7 | 7.5 |
| CC(1/2) | 0.998 | 0.882 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | PEG MME 550, Potassium nitrate, Magnesium Nitrate, HEPES |
