4ZRK

Merlin-FERM and Lats1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-01-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	111.050, 110.774, 168.693
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.780 - 2.316
R-factor	0.2474
Rwork	0.246
R-free	0.27860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1isn
RMSD bond length	0.010
RMSD bond angle	1.232
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge		0.775
Number of reflections	89074
<I/σ(I)>	28.1	2.5
Completeness [%]	99.4	99.8
Redundancy	5.9	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.1 M Sodium malonate, 10% PEG 3350