4ZRG
Visual arrestin mutant - R175E
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-19 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97902 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.373, 72.314, 79.405 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.637 - 2.700 |
| R-factor | 0.1961 |
| Rwork | 0.194 |
| R-free | 0.24140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ugx |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.006 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.640 | 2.830 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.217 | 0.924 |
| Number of reflections | 10993 | |
| <I/σ(I)> | 10.8 | |
| Completeness [%] | 99.9 | 99.91 |
| Redundancy | 7.6 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 292.15 | 100mM Tris, 8-18% (w/v) PEG 4000 |
