4ZRE
Crystal structure of SMG1 F278D mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | OXFORD DIFFRACTION ENHANCE ULTRA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-06-06 |
Detector | OXFORD RUBY CCD |
Wavelength(s) | 1.54056 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.740, 73.239, 44.919 |
Unit cell angles | 90.00, 108.51, 90.00 |
Refinement procedure
Resolution | 18.400 - 2.000 |
Rwork | 0.157 |
R-free | 0.20480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3uue |
Data reduction software | CrysalisPro |
Data scaling software | Aimless (0.2.8) |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 18.410 | 18.410 | 2.050 |
High resolution limit [Å] | 2.000 | 8.720 | 2.000 |
Rmerge | 0.134 | 0.075 | 0.331 |
Rpim | 0.065 | 0.053 | 0.195 |
Total number of observations | 84074 | 537 | 4713 |
Number of reflections | 16530 | ||
<I/σ(I)> | 12.3 | 44.7 | 3.5 |
Completeness [%] | 99.8 | 89.3 | 98.8 |
Redundancy | 5.1 | 2.8 | 3.7 |
CC(1/2) | 0.989 | 0.978 | 0.887 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.3 | 277 | 0.1M Bis-Tris pH5.3, 20% PEG 3350 |