4ZRE

Crystal structure of SMG1 F278D mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	OXFORD DIFFRACTION ENHANCE ULTRA
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-06
Detector	OXFORD RUBY CCD
Wavelength(s)	1.54056
Spacegroup name	P 1 21 1
Unit cell lengths	39.740, 73.239, 44.919
Unit cell angles	90.00, 108.51, 90.00

Refinement procedure

Resolution	18.400 - 2.000
Rwork	0.157
R-free	0.20480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3uue
Data reduction software	CrysalisPro
Data scaling software	Aimless (0.2.8)
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	18.410	18.410	2.050
High resolution limit [Å]	2.000	8.720	2.000
Rmerge	0.134	0.075	0.331
Rpim	0.065	0.053	0.195
Total number of observations	84074	537	4713
Number of reflections	16530
<I/σ(I)>	12.3	44.7	3.5
Completeness [%]	99.8	89.3	98.8
Redundancy	5.1	2.8	3.7
CC(1/2)	0.989	0.978	0.887

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.3	277	0.1M Bis-Tris pH5.3, 20% PEG 3350