4ZRD

Crystal structure of SMG1 F278N mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9952
Spacegroup name	P 43
Unit cell lengths	77.310, 77.310, 61.060
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.000 - 2.300
Rwork	0.175
R-free	0.22910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3uue
Data scaling software	Aimless (0.2.8)
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.920	47.920	2.380
High resolution limit [Å]	2.300	8.910	2.300
Rmerge	0.103	0.059	0.468
Rpim	0.067	0.040	0.303
Total number of observations	52161	889	5150
Number of reflections	16070
<I/σ(I)>	7	13.2	2.3
Completeness [%]	99.6	96.8	99.9
Redundancy	3.2	3.1	3.3
CC(1/2)	0.989	0.990	0.783

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	5.3	277	0.1M Bis-Tris pH6.3, 28%PEG3350