4ZQY

Ringhalexin from hemachatus haemachatus: A novel inhibitor of extrinsic tenase complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	200
Detector technology	CCD
Collection date	2012-05-11
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.54178
Spacegroup name	P 41 21 2
Unit cell lengths	82.160, 82.160, 82.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.951
R-factor	0.2197
Rwork	0.217
R-free	0.26800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2h8u
RMSD bond length	0.009
RMSD bond angle	1.599
Data reduction software	MOSFLM
Data scaling software	MOSFLM
Phasing software	BALBES
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall
Low resolution limit [Å]	15.000
High resolution limit [Å]	2.951
Number of reflections	6253
<I/σ(I)>	14.7
Completeness [%]	99.4
Redundancy	8.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	29% MPD, 0.1M HEPES