4ZQQ

Apo form of influenza strain H1N1 polymerase acidic subunit N-terminal region

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-27
Detector	ADSC QUANTUM 270
Wavelength(s)	1.000
Spacegroup name	P 41 21 2
Unit cell lengths	66.333, 66.333, 127.338
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.415 - 1.800
R-factor	0.2262
Rwork	0.224
R-free	0.27390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m5q
RMSD bond length	0.010
RMSD bond angle	1.225
Data scaling software	HKL-2000
Phasing software	MOLREP (11.0.05)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.730
High resolution limit [Å]	1.700	4.610	1.700
Rmerge	0.072	0.061	0.900
Rmeas	0.075	0.065	0.932
Rpim	0.021	0.022	0.242
Total number of observations	423428
Number of reflections	30558
<I/σ(I)>	13.3
Completeness [%]	95.1	57.2	99.4
Redundancy	13.9	8	14.4
CC(1/2)		0.994	0.889

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.8	291	100mM MES, 1.1M ammonium sulfate, 0.1M potassium chloride, 9% trehalose