4ZQ3
Crystal structure of Staphylococcal nuclease variant Delta+PHS I92T at cryogenic temperature
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | OTHER |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2014-04-16 |
Detector | APEX II CCD |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 31.085, 60.217, 38.037 |
Unit cell angles | 90.00, 93.03, 90.00 |
Refinement procedure
Resolution | 32.130 - 1.900 |
R-factor | 0.1897 |
Rwork | 0.187 |
R-free | 0.25350 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3bdc |
RMSD bond length | 0.018 |
RMSD bond angle | 1.806 |
Data reduction software | XPREP |
Data scaling software | SAINT |
Phasing software | PHASER (2.5.1) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.029 | |
Total number of observations | 117365 | |
Number of reflections | 11102 | |
<I/σ(I)> | 21.6 | 4.52 |
Completeness [%] | 99.7 | 98.2 |
Redundancy | 10.13 | 5.62 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 277 | 35% MPD, 25 mM potassium phosphate, calcium chloride, pdTp |