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4ZQ3

Crystal structure of Staphylococcal nuclease variant Delta+PHS I92T at cryogenic temperature

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSEALED TUBE
Source detailsOTHER
Temperature [K]110
Detector technologyCCD
Collection date2014-04-16
DetectorAPEX II CCD
Wavelength(s)1.54
Spacegroup nameP 1 21 1
Unit cell lengths31.085, 60.217, 38.037
Unit cell angles90.00, 93.03, 90.00
Refinement procedure
Resolution32.130 - 1.900
R-factor0.1897
Rwork0.187
R-free0.25350
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)3bdc
RMSD bond length0.018
RMSD bond angle1.806
Data reduction softwareXPREP
Data scaling softwareSAINT
Phasing softwarePHASER (2.5.1)
Refinement softwareREFMAC
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0001.930
High resolution limit [Å]1.9001.900
Rmerge0.029
Total number of observations117365
Number of reflections11102
<I/σ(I)>21.64.52
Completeness [%]99.798.2
Redundancy10.135.62
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP927735% MPD, 25 mM potassium phosphate, calcium chloride, pdTp

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