4ZPY

Structure of N170A MVM mutant empty capsid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-07-22
Detector	PSI PILATUS 6M
Wavelength(s)	0.9787
Spacegroup name	H 3 2
Unit cell lengths	410.190, 410.190, 559.700
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.900 - 3.800
Rwork	0.282
R-free	0.28910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1z14
Data scaling software	Aimless (0.5.8)
Phasing software	PHASER
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.930	49.930	3.870
High resolution limit [Å]	3.800	20.810	3.800
Rmerge	0.200	0.076	0.789
Number of reflections	176252
<I/σ(I)>	7.7	24.5	2.4
Completeness [%]	99.8	93	97.7
Redundancy	10.5	10.3	9.9
CC(1/2)	0.982	0.985	0.766

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	MPD, NaCl, NaAc