4ZPX
Crystal structure of Lon ATPase domain from Thermococcus onnurineus NA1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-11 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.620, 61.599, 76.321 |
| Unit cell angles | 74.61, 87.11, 83.45 |
Refinement procedure
| Resolution | 41.140 - 2.030 |
| R-factor | 0.18444 |
| Rwork | 0.182 |
| R-free | 0.23870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.856 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.030 |
| Number of reflections | 42270 |
| <I/σ(I)> | 23.03 |
| Completeness [%] | 93.1 |
| Redundancy | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 295 | 0.2M Potassium chloride, 0.01M Magnesium acetate, 0.05M tri-sodium citrate dihydrate pH 4.5, 12% PEG 4000, 5% n-Dodecyl-beta-D-maltoside |
