4ZPS
Crystal Structure of Protocadherin Gamma A8 EC1-3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 64.060, 78.120, 167.730 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.651 - 2.900 |
| R-factor | 0.2657 |
| Rwork | 0.264 |
| R-free | 0.28650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.610 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 3.080 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.080 | 0.400 |
| Number of reflections | 9489 | |
| <I/σ(I)> | 10.2 | 3.2 |
| Completeness [%] | 98.9 | 96.4 |
| Redundancy | 3.1 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.7 | 295 | 28% PEG400, 100mM Tris-Cl, pH 8.7 |
