4ZPQ
Crystal Structure of Protocadherin Gamma C5 EC1-3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 190.806, 104.916, 80.066 |
| Unit cell angles | 90.00, 97.03, 90.00 |
Refinement procedure
| Resolution | 29.192 - 3.099 |
| R-factor | 0.2167 |
| Rwork | 0.215 |
| R-free | 0.25230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zpo |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.585 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.210 |
| High resolution limit [Å] | 3.099 | 3.100 |
| Rmerge | 0.110 | 0.390 |
| Number of reflections | 26871 | |
| <I/σ(I)> | 10 | 2.5 |
| Completeness [%] | 94.4 | 76.2 |
| Redundancy | 3.5 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 16% (w/v) PEG 6000, 200mM calcium acetate, 100mM imidazole, pH 8.0 |
