4ZPP
Crystal Structure of Protocadherin Gamma C5 EC1-3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-07-19 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 67.188, 84.563, 109.144 |
Unit cell angles | 90.00, 106.43, 90.00 |
Refinement procedure
Resolution | 27.938 - 3.002 |
R-factor | 0.2115 |
Rwork | 0.209 |
R-free | 0.24810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4zpo |
RMSD bond length | 0.003 |
RMSD bond angle | 0.594 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.070 | 0.390 |
Number of reflections | 23383 | |
<I/σ(I)> | 16.8 | 2.8 |
Completeness [%] | 99.1 | 93.5 |
Redundancy | 3.7 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 25.5% (w/v) PEG 4000, 15% (v/v) glycerol, 3mM calcium chloride, 85mM Tris-Cl, pH 8.5 |