4ZPP
Crystal Structure of Protocadherin Gamma C5 EC1-3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.188, 84.563, 109.144 |
| Unit cell angles | 90.00, 106.43, 90.00 |
Refinement procedure
| Resolution | 27.938 - 3.002 |
| R-factor | 0.2115 |
| Rwork | 0.209 |
| R-free | 0.24810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zpo |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.594 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.070 | 0.390 |
| Number of reflections | 23383 | |
| <I/σ(I)> | 16.8 | 2.8 |
| Completeness [%] | 99.1 | 93.5 |
| Redundancy | 3.7 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 25.5% (w/v) PEG 4000, 15% (v/v) glycerol, 3mM calcium chloride, 85mM Tris-Cl, pH 8.5 |
