4ZPO
Crystal Structure of Protocadherin Gamma C5 EC1-3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-08-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.98 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 108.637, 108.637, 96.614 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.064 - 2.900 |
R-factor | 0.2216 |
Rwork | 0.220 |
R-free | 0.24720 |
Structure solution method | MIR |
RMSD bond length | 0.002 |
RMSD bond angle | 0.514 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.064 | 3.080 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.100 | 0.360 |
Number of reflections | 13086 | |
<I/σ(I)> | 19.8 | 5.7 |
Completeness [%] | 99.9 | 100 |
Redundancy | 14.2 | 14.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | 40% (v/v) MPD, 5% (w/v) PEG 8000, 100mM sodium cacodylate, pH 6 |