4ZPM
Crystal Structure of Protocadherin Alpha C2 EC1-3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-09-26 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 24.990, 97.130, 147.680 |
| Unit cell angles | 90.00, 94.18, 90.00 |
Refinement procedure
| Resolution | 31.620 - 2.400 |
| R-factor | 0.2099 |
| Rwork | 0.208 |
| R-free | 0.25170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zpo |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.730 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.620 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.080 | 0.460 |
| Number of reflections | 26357 | |
| <I/σ(I)> | 9.1 | 1.6 |
| Completeness [%] | 95.8 | 93.4 |
| Redundancy | 3 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 295 | 28% PEG MME 500, 100mM sodium acetate, pH 4 |
