4ZPL
Crystal Structure of Protocadherin Beta 1 EC1-3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 74.990, 106.520, 149.350 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.696 - 3.300 |
| R-factor | 0.2336 |
| Rwork | 0.231 |
| R-free | 0.27800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zpo |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.742 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.700 | 3.560 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.150 | 0.490 |
| Number of reflections | 9021 | |
| <I/σ(I)> | 4.6 | 1.6 |
| Completeness [%] | 97.9 | 97.5 |
| Redundancy | 2.6 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 24% (w/v) PEG1500, 20% (v/v) glycerol, 3% (w/v) glucose |
