4ZP7

Coxsackievirus B3 Polymerase - F364V mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-31
Detector	ADSC QUANTUM 210
Wavelength(s)	1.00
Spacegroup name	P 43 21 2
Unit cell lengths	74.571, 74.571, 289.828
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	52.730 - 1.901
R-factor	0.1886
Rwork	0.188
R-free	0.21990
RMSD bond length	0.007
RMSD bond angle	0.823
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	52.730		2.020
High resolution limit [Å]	1.901	5.680	1.900
Rmerge	0.110	0.054	0.605
Rmeas	0.118	0.058	0.664
Total number of observations	863218
Number of reflections	122509	4610	19552
<I/σ(I)>	11.92	27.78	2.17
Completeness [%]	99.7	99.9	98.1
Redundancy	13.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	289	0.085 M Tris, 1.2 M Ammonium Sulfate , 25% Glycerol