4ZP3
AKAP18:PKA-RIIalpha structure reveals crucial anchor points for recognition of regulatory subunits of PKA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.922, 120.988, 57.123 |
| Unit cell angles | 90.00, 93.01, 90.00 |
Refinement procedure
| Resolution | 41.500 - 2.630 |
| R-factor | 0.22452 |
| Rwork | 0.222 |
| R-free | 0.26770 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.971 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 41.500 |
| High resolution limit [Å] | 2.630 |
| Number of reflections | 22903 |
| <I/σ(I)> | 10.61 |
| Completeness [%] | 99.4 |
| Redundancy | 3.25 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG3350, Cadmium chloride, Sodium acetate |
