4ZOZ
Crystal structure of the Chaetomium thermophilum Sqt1 bound to the N-terminus of the ribosomal protein L10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-08 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.03320 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.497, 67.178, 72.815 |
| Unit cell angles | 89.92, 108.92, 92.03 |
Refinement procedure
| Resolution | 48.330 - 1.700 |
| R-factor | 0.1925 |
| Rwork | 0.191 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zoy |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.090 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1685) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 48.330 |
| High resolution limit [Å] | 1.700 |
| Number of reflections | 87711 |
| <I/σ(I)> | 13.6 |
| Completeness [%] | 93.7 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M sodium chloride, 0.1 M Na/K-phosphate pH 6.2, 40% PEG400 |
