4ZOZ
Crystal structure of the Chaetomium thermophilum Sqt1 bound to the N-terminus of the ribosomal protein L10
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-10-08 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.03320 |
Spacegroup name | P 1 |
Unit cell lengths | 47.497, 67.178, 72.815 |
Unit cell angles | 89.92, 108.92, 92.03 |
Refinement procedure
Resolution | 48.330 - 1.700 |
R-factor | 0.1925 |
Rwork | 0.191 |
R-free | 0.21600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4zoy |
RMSD bond length | 0.007 |
RMSD bond angle | 1.090 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1685) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 48.330 |
High resolution limit [Å] | 1.700 |
Number of reflections | 87711 |
<I/σ(I)> | 13.6 |
Completeness [%] | 93.7 |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M sodium chloride, 0.1 M Na/K-phosphate pH 6.2, 40% PEG400 |