4ZOY

Crystal structure of the Chaetomium thermophilum Sqt1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-09-27
Detector	PSI PILATUS 6M
Wavelength(s)	1.03320
Spacegroup name	P 21 21 21
Unit cell lengths	67.440, 73.020, 113.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.377 - 1.500
R-factor	0.1846
Rwork	0.184
R-free	0.20190
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.092
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.9_1685)

Data quality characteristics

	Overall
Low resolution limit [Å]	37.380
High resolution limit [Å]	1.500
Number of reflections	89196
<I/σ(I)>	11.2
Completeness [%]	98.7
Redundancy	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M Tris pH 8.5, 20% (v/v) EtOH