4ZOX
Crystal structure of the Saccharomyces cerevisiae Sqt1 bound to the N-terminus of the ribosomal protein L10
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-04-13 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.97239 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 74.273, 89.252, 53.559 |
Unit cell angles | 90.00, 100.28, 90.00 |
Refinement procedure
Resolution | 44.626 - 1.600 |
R-factor | 0.1479 |
Rwork | 0.146 |
R-free | 0.18060 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4zov |
RMSD bond length | 0.007 |
RMSD bond angle | 1.127 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1685) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 44.630 |
High resolution limit [Å] | 1.600 |
Number of reflections | 44971 |
<I/σ(I)> | 18.5 |
Completeness [%] | 99.4 |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M Ca-acetate, 0.1 M Nacacodylate pH 6.5, 40% (v/v) PEG600 |