4ZOX
Crystal structure of the Saccharomyces cerevisiae Sqt1 bound to the N-terminus of the ribosomal protein L10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-13 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97239 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 74.273, 89.252, 53.559 |
| Unit cell angles | 90.00, 100.28, 90.00 |
Refinement procedure
| Resolution | 44.626 - 1.600 |
| R-factor | 0.1479 |
| Rwork | 0.146 |
| R-free | 0.18060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zov |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.127 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1685) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 44.630 |
| High resolution limit [Å] | 1.600 |
| Number of reflections | 44971 |
| <I/σ(I)> | 18.5 |
| Completeness [%] | 99.4 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M Ca-acetate, 0.1 M Nacacodylate pH 6.5, 40% (v/v) PEG600 |
