4ZOW
Crystal structure of E. coli multidrug transporter MdfA in complex with chloramphenicol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 93.852, 63.449, 99.726 |
| Unit cell angles | 90.00, 106.09, 90.00 |
Refinement procedure
| Resolution | 45.088 - 2.450 |
| R-factor | 0.2055 |
| Rwork | 0.204 |
| R-free | 0.23540 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.844 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | PHENIX (phenix.refine: 1.8_1069) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.540 |
| High resolution limit [Å] | 2.450 | 5.280 | 2.450 |
| Rmerge | 0.116 | 0.066 | 0.996 |
| Total number of observations | 136611 | ||
| Number of reflections | 21233 | ||
| <I/σ(I)> | 6.7 | ||
| Completeness [%] | 99.6 | 99.8 | 97.3 |
| Redundancy | 6.4 | 6.7 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 295 | sodium acetate, PEG 400, magnesium acetate |
