4ZOV
Crystal structure of the Saccharomyces cerevisiae Sqt1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-12 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.87260 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.793, 75.165, 101.254 |
| Unit cell angles | 90.00, 104.42, 90.00 |
Refinement procedure
| Resolution | 42.565 - 2.000 |
| R-factor | 0.1882 |
| Rwork | 0.187 |
| R-free | 0.22290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.210 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1685) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 42.570 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.036 |
| Number of reflections | 51857 |
| <I/σ(I)> | 16.5 |
| Completeness [%] | 98.5 |
| Redundancy | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 95 mM Na-citrate pH 5.6, 19% (v/v) isopropanol, 19% (w/v) PEG4000, 5% glycerol |
