4ZOS
2.20 Angstrom resolution crystal structure of protein YE0340 of unidentified function from Yersinia enterocolitica subsp. enterocolitica 8081]
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-22 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 1 |
| Unit cell lengths | 35.675, 37.135, 82.583 |
| Unit cell angles | 87.66, 88.25, 61.17 |
Refinement procedure
| Resolution | 29.660 - 2.200 |
| R-factor | 0.23165 |
| Rwork | 0.229 |
| R-free | 0.28529 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1x7v |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.623 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.070 | 0.337 |
| Number of reflections | 18325 | |
| <I/σ(I)> | 16.5 | 3.2 |
| Completeness [%] | 96.1 | 90 |
| Redundancy | 3.7 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | Crystallization: 20%Polyethylene Glycol 8000, 0.2M Sodium Chloride,Phosphate-Citrate Protein at 20 mg/ml |
