4ZOR
The structure of the S37P MS2 viral capsid assembly.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-08-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97 |
| Spacegroup name | F 2 3 |
| Unit cell lengths | 228.125, 228.125, 228.125 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.900 - 2.200 |
| R-factor | 0.219 |
| Rwork | 0.218 |
| R-free | 0.24250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ms2 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.989 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (DEV_1839) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.900 | 2.281 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.145 | 1.577 |
| Number of reflections | 49435 | |
| <I/σ(I)> | 7.7 | 1.02 |
| Completeness [%] | 99.9 | 0.99 |
| Redundancy | 5 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 299 | 1.8M lithium sulphate, 100mM TRIS, pH 7.55, 20% glycerol (cryo) |
