4ZOH
Crystal structure of glyceraldehyde oxidoreductase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL40B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL40B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-02 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 143.431, 143.431, 235.509 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.400 - 2.200 |
| R-factor | 0.17998 |
| Rwork | 0.177 |
| R-free | 0.24231 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ffv |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.098 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.394 | |
| Number of reflections | 73074 | |
| <I/σ(I)> | 36.7 | 6.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.1 | 12.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 0.1 MNa-acetate, 0.2M CH3COONH4, 15% (w/v) PEG 4000 |
