4ZOC
Crystal Structure of mutant (D270A) beta-glucosidase from Listeria innocua in complex with sophorotriose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-05-26 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 89.620, 94.860, 215.065 |
| Unit cell angles | 90.00, 96.33, 90.00 |
Refinement procedure
| Resolution | 32.500 - 1.790 |
| R-factor | 0.1438 |
| Rwork | 0.141 |
| R-free | 0.19600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zoe |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.938 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 32.500 |
| High resolution limit [Å] | 1.790 |
| Number of reflections | 166046 |
| <I/σ(I)> | 8.4 |
| Completeness [%] | 99.0 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | PEG 4000, NaCl, LiSO4, glycerol |
