4ZO6
Crystal Structure of mutant (D270A) beta-glucosidase from Listeria innocua in complex with cellobiose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-05-26 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0 |
Spacegroup name | I 1 2 1 |
Unit cell lengths | 89.790, 95.350, 215.376 |
Unit cell angles | 90.00, 96.30, 90.00 |
Refinement procedure
Resolution | 32.560 - 2.000 |
R-factor | 0.1868 |
Rwork | 0.183 |
R-free | 0.25490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4zoe |
RMSD bond length | 0.016 |
RMSD bond angle | 1.834 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 32.560 |
High resolution limit [Å] | 2.000 |
Number of reflections | 117731 |
<I/σ(I)> | 5.6 |
Completeness [%] | 97.0 |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | PEG 4000, NaCl, LiSO4, glycerol |