4ZO4
Dephospho-CoA kinase from Campylobacter jejuni.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.815, 74.032, 178.493 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.240 - 2.570 |
| R-factor | 0.2113 |
| Rwork | 0.208 |
| R-free | 0.27760 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.421 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.610 |
| High resolution limit [Å] | 2.570 | 6.970 | 2.570 |
| Rmerge | 0.091 | 0.056 | 0.799 |
| Total number of observations | 187665 | ||
| Number of reflections | 32010 | ||
| <I/σ(I)> | 9.8 | 2.15 | |
| Completeness [%] | 99.8 | 97.3 | 100 |
| Redundancy | 5.9 | 5.2 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 289 | 2 M sodium/potassium phosphate buffer, 0.1 M phosphate citrate buffer |
