4ZNY

Structure of the human TSG101-UEV Domain in complex with the PTAP motif of the p19 gag protein of the Human T-cell Leukemia type I virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-06-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97779
Spacegroup name	C 2 2 21
Unit cell lengths	78.622, 119.743, 41.641
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.957 - 2.400
R-factor	0.2306
Rwork	0.230
R-free	0.24310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4eje
RMSD bond length	0.003
RMSD bond angle	0.842
Data reduction software	XDS
Data scaling software	Aimless (0.5.8)
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.960	19.960	2.490
High resolution limit [Å]	2.400	8.980	2.400
Rmerge	0.032	0.020	0.279
Rpim	0.017	0.012	0.134
Total number of observations	37420	635	4376
Number of reflections	8020
<I/σ(I)>	34.6	62.6	9.7
Completeness [%]	99.8	91.2	100
Redundancy	4.7	3.8	5.3
CC(1/2)	0.999	0.999	0.977

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	298	30% PEG4k, 0.1 ammonium sulfate, 0.1 MES