4ZNQ
Crystal structure of Dln1 complexed with Man(alpha1-2)Man
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-05-12 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97915 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.169, 95.553, 147.707 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.610 - 1.900 |
R-factor | 0.18267 |
Rwork | 0.181 |
R-free | 0.21853 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.424 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.095 | 0.413 |
Number of reflections | 63944 | |
<I/σ(I)> | 12 | 3.3 |
Completeness [%] | 99.7 | 100 |
Redundancy | 3.9 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 15%(w/v) PEG 6000, 0.1M HEPES pH 7.5, 1.0M LiCl |