4ZNP
The structure of A pfI Riboswitch Bound to ZMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 152.195, 152.195, 86.891 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.200 - 2.940 |
| R-factor | 0.2244 |
| Rwork | 0.223 |
| R-free | 0.24840 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.394 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELX |
| Refinement software | PHENIX (dev_1839) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 43.200 |
| High resolution limit [Å] | 2.940 |
| Number of reflections | 13111 |
| <I/σ(I)> | 34.9 |
| Completeness [%] | 99.9 |
| Redundancy | 19.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 0.1 M Na-acetate, pH 4.6, 0.2 M (NH4)2SO4 and 20-30% PEG4000/PEG2000MEM |
