4ZNK

Thermus Phage P74-26 Large Terminase ATPase domain from (P 32 2 1 space group)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-07
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.033
Spacegroup name	P 32 2 1
Unit cell lengths	62.890, 62.890, 133.100
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.398 - 1.931
R-factor	0.153
Rwork	0.151
R-free	0.19650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zni
RMSD bond length	0.009
RMSD bond angle	1.173
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX (1.9_1692)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.400	2.000
High resolution limit [Å]	1.931	1.931
Rmerge	0.090	0.504
Number of reflections	23556
<I/σ(I)>	10.71	2.96
Completeness [%]	99.7	97.75
Redundancy	4.8	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	298	11% (w/v) PEG 2000, 0.15 M ammonium sulfate, and 0.1 M tri-sodium citrate pH 5.0